Temporal regulation of lin-14 by the antagonistic action of two other heterochronic genes, lin-4 and lin-28.
نویسندگان
چکیده
منابع مشابه
Investigation of lin-4 independent regulation of lin-28 in the C. elegans heterochronic
developmental timing pathway. (1) SPECIFIC AIMS Appropriate temporal patterning is essential for embryogenesis and post-embryonic development. Progression through the four larval stages in C. elegans is regulated by 'heterochronic' genes (reviewed in Slack and Ruvkun 1997; Ambros 2000). Heterochronic genes encode a diverse set of proteins, mutations in which result in the reiteration or omissio...
متن کاملPosttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans.
During C. elegans development, the temporal pattern of many cell lineages is specified by graded activity of the heterochronic gene Lin-14. Here we demonstrate that a temporal gradient in Lin-14 protein is generated posttranscriptionally by multiple elements in the lin-14 3'UTR that are regulated by the heterochronic gene Lin-4. The lin-14 3'UTR is both necessary and sufficient to confer lin-4-...
متن کاملThe C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate ...
متن کاملDual Regulation of the lin-14 Target mRNA by the lin-4 miRNA
microRNAs (miRNAs) are ∼22 nt regulatory RNAs that in animals typically bind with partial complementarity to sequences in the 3' untranslated (UTR) regions of target mRNAs, to induce a decrease in the production of the encoded protein. The relative contributions of translational inhibition of intact mRNAs and degradation of mRNAs caused by binding of the miRNA vary; for many genetically validat...
متن کاملMolecular genetics of the Caenorhabditis elegans heterochronic gene lin-14.
We describe a general strategy for the genetic mapping in parallel of multiple restriction fragment length polymorphism (RFLP) loci. This approach allows the systematic identification for cloning of physical genetic loci within about 100 kb of any gene in Caenorhabditis elegans. We have used this strategy of parallel RFLP mapping to clone the heterochronic gene lin-14, which controls the timing...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 1991
ISSN: 0890-9369
DOI: 10.1101/gad.5.10.1825